Rabbit Monoclonal Antibody to Phospho-MCM2 (Ser27)

包装: 1G|100G|500G|1KG|100KG

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别名:MCM2; BM28; CCNL1; CDCL1; KIAA0030; DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28
应用:WB,IHC,ICC,IP
反应种属:Human
规格：50μl/100μl

Description
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]

Description

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]

Specification
Aliases	MCM2; BM28; CCNL1; CDCL1; KIAA0030; DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28
Entrez GeneID	4171
Swissprot	P49736
WB Predicted band size	Calculated MW: 102 kDa; Observed MW: 125 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	A synthetic phosphopeptide corresponding to residues surrounding Ser27 of human MCM2
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Application
IP	1/20
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200

	Western blot analysis of MCM2 (Phospho-Ser27) in Hela, A549, HL-60, U2OS lysates using MCM2 (Phospho-Ser27) antibody.
	Immunocytochemistry analysis of Phospho-MCM2 (Ser27) (green) in Hela using Phospho-MCM2 (Ser27) antibody,and DAPI(blue).
	Immunohistochemistry analysis of paraffin-embedded Human tonsil using MCM2 (Phospho-Ser27) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.