Rabbit Monoclonal Antibody to Phospho-Rb (Thr356)

包装: 1G|100G|500G|1KG|100KG

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别名:RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110
应用:WB,IHC,IP
反应种属:Human,Rat
规格：50μl/100μl

Description
Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.

Description

Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.

Specification
Aliases	RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110
Entrez GeneID	5925
Swissprot	P06400
WB Predicted band size	Calculated MW: 106 kDa; Observed MW: 110 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Rat
Immunogen	A synthetic phosphopeptide corresponding to residues surrounding Thr356 of human Rb
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Application
IP	1/20
WB	1/500-1/1000
IHC	1/50-1/100

	Western blot analysis of Rb (Phospho-Thr356) in HL-60, U2OS, C6 lysates using Phospho-Rb (Thr356) antibody.
	Immunohistochemistry analysis of paraffin-embedded Human tonsil using Rb (Phospho-Thr356) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.