Rabbit Monoclonal Antibody to Phospho-Rb2 p130 (Ser952)

包装: 1G|100G|500G|1KG|100KG

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别名:Rb2; P130
应用:WB,IHC,ICC
反应种属:Human
规格：50μl/100μl

Description
Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 ‘Lys-20’ trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.

Description

Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 ‘Lys-20’ trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.

Specification
Aliases	Rb2; P130
Entrez GeneID	5934
Swissprot	Q08999
WB Predicted band size	Calculated MW: 128 kDa; Observed MW: 128 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	A synthetic phosphopeptide corresponding to residues surrounding Ser952 of human Rb2 p130
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Application
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200

	Western blot analysis of Rb2 p130 (Phospho-Ser952) in K562, Hela lysates using Phospho-Rb2 p130 (Ser952) antibody.
	Immunocytochemistry analysis of Rb2 p130 (Phospho-Ser952)(green) in Hela using Rb2 p130 (Phospho-Ser952) antibody,and DAPI(blue)
	Immunohistochemistry analysis of paraffin-embedded Human tonsil using Rb2 p130 (Phospho-Ser952) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.