Rabbit Monoclonal Antibody to Phospho-Smad2 (Ser255)

包装: 1G|100G|500G|1KG|100KG

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别名:SMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2
应用:WB,IHC,IP
反应种属:Human
规格：50μl/100μl

Description
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene ‘mothers against decapentaplegic’ (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.

Specification
Aliases	SMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2
Entrez GeneID	4087
Swissprot	Q15796
WB Predicted band size	Calculated MW: 52 kDa; Observed MW: 60 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	A synthetic phosphopeptide corresponding to residues surrounding Ser255 of human Smad2
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Application
IP	1/20
WB	1/500-1/1000
IHC	1/50-1/100

	Western blot analysis of Phospho-Smad2 (Ser255) in K562 lysates using Phospho-Smad2 (Ser255) antibody.
	Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Phospho-Smad2 (Ser255) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.