Rabbit Monoclonal Antibody to POLD1

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别名:CDC2; CRCS10; MDPL; POLD; POLD1
应用:WB,ICC
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3′- to 5′-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex.

Description

Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3′- to 5′-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex.

Specification
Aliases	CDC2; CRCS10; MDPL; POLD; POLD1
Entrez GeneID	5424
Swissprot	P28340
WB Predicted band size	Calculated MW: 124 kDa; Observed MW: 124 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	A synthetic peptide of human DNA Polymerase delta, catalytic subunit
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Application
WB	1/500-1/1000
ICC	1/50-1/200

	Western blot analysis of POLD1 in K562, C6, 3T3, Hela lysates using POLD1 antibody.
	Immunocytochemistry analysis of POLD1 (green) in K562 using POLD1 antibody,and DAPI(blue).