Rabbit Monoclonal Antibody to TATA Box Binding Protein

包装: 1G|100G|500G|1KG|100KG

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别名:TBP; GTF2D1; TF2D; TFIID; TATA-box-binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box factor; Transcription initiation factor TFIID TBP subunit
应用:WB,IHC,ICC,IP
反应种属:Human,Mouse
规格：50μl/100μl

Description
As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This protein is not suitable for samples where the nuclear envelope has been removed.

Description

As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This protein is not suitable for samples where the nuclear envelope has been removed.

Specification
Aliases	TBP; GTF2D1; TF2D; TFIID; TATA-box-binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box factor; Transcription initiation factor TFIID TBP subunit
Entrez GeneID	6908
Swissprot	P20226
WB Predicted band size	Calculated MW: 38 kDa; Observed MW: 38 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse
Immunogen	A synthetic peptide of human TATA binding protein TBP
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Application
IP	1/20
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200

	Western blot analysis of TATA binding protein TBP in 3T3 lysates using TATA Box Binding Protein antibody.
	Western blot analysis of TATA binding protein TBP in Hela, A549, HL-60, U2OS lysates using TATA binding protein TBP antibody.
	Immunocytochemistry analysis of TATA binding protein TBP (green) in hela using TATA binding protein TBP antibody,and DAPI(blue).
	Immunohistochemistry analysis of paraffin-embedded Human tonsil using TATA binding protein TBP antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.