Rabbit Monoclonal Antibody to Ubiquitin K48

包装: 1G|100G|500G|1KG|100KG

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别名:FLJ25987; MGC8385; ubiquitin B; Ubiquitin; UBCEP1; UBCEP2; RPS27A
应用:WB,IHC,ICC
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue.

Description

Plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue.

Specification
Aliases	FLJ25987; MGC8385; ubiquitin B; Ubiquitin; UBCEP1; UBCEP2; RPS27A
Entrez GeneID	7314
Swissprot	P0CG47
WB Predicted band size	Refer to figures
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	A synthetic peptide of human K-48 Linkage Specific Ubiquitin
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Application
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200

	Western blot analysis of K48 Linkage Specific Ubiquitin in C6, C2C12 lysates using Ubiquitin K48 antibody.
	Western blot analysis of K48 Linkage Specific Ubiquitin in 293 lysates using K48 Linkage Specific Ubiquitin antibody.
	Immunohistochemistry analysis of paraffin-embedded Human colon cancer using K48 Linkage Specific Ubiquitin antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.