Rabbit Polyclonal Antibody to 4E BP1

包装: 1G|100G|500G|1KG|100KG

现货支持定制

QQ咨询
| 微信咨询

满200元免运费
正品保障提供发票
现货闪电当时发货

别名:EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
应用:WB,IHC,ICC,FCM,IP
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity.

Specification
Aliases	EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Entrez GeneID	1978
Swissprot	Q13541
WB Predicted band size	Calculated MW: 13 kDa; Observed MW: 18 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	A synthesized peptide derived from human eIF4EBP1
Formulation	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
IP	1/20
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200
FCM	1/50-1/100

	Western blot analysis of eIF4EBP1 in K562 lysates using 4E BP1 antibody.
	Immunofluorescence analysis of 4E BP1 in HeLa using eIF4EBP1 antibody.
	Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using eIF4EBP1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.