Rabbit Polyclonal Antibody to 4E-BP1 (Ab-64)

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别名:4EBP1; EIF4EBP1; P/OKCL.6; PHAS-1;
应用:WB,IHC
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Regulates eIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.Anne-Claude Gingras, Genes & Dev., Jun 1999; 13: 1422.Guangming Liu, J. Biol. Chem., Mar 2002; 277: 8810 – 8816.Ya-Ping Jiang, J. Biol. Chem., Mar 2001; 276: 10943 – 10951.

Description

Regulates eIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.Anne-Claude Gingras, Genes & Dev., Jun 1999; 13: 1422.Guangming Liu, J. Biol. Chem., Mar 2002; 277: 8810 – 8816.Ya-Ping Jiang, J. Biol. Chem., Mar 2001; 276: 10943 – 10951.

Specification
Aliases	4EBP1; EIF4EBP1; P/OKCL.6; PHAS-1;
Entrez GeneID	1978;
Swissprot	Q13541
WB Predicted band size	15kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Synthesized non-phosphopeptide derived from human 4E-BP1 around the phosphorylation site of serine 65 (R-N-S(p)-P-V).
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100

	Western blot analysis of extracts from Jurkat cells, treated with Insulin (0.01U/ml, 15mins), using 4E-BP1 (Ab-64) antibody P41640.
	Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using 4E-BP1 (Ab-64) antibody P41640.