中文 
别名:6 His epitope tag应用:WB
反应种属:Fusion proteins containing the HIS Tag
规格：50μl/100μl
| Description |
|---|
| The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification. It can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting. The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells. |
| Specification | |
|---|---|
| Aliases | 6 His epitope tag |
| WB Predicted band size | 50/44 kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Fusion proteins containing the HIS Tag |
| Immunogen | Synthetic peptide: 18×His conjugated to KLH |
| Formulation | pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
| Application | |
|---|---|
| WB | 1/2000-1/10000 |
| ELISA | 1/5000-1/10000 |
 |
Gel: 8%SDS-PAGE, Lysate: 1/0.5/0.5 μg, Lane 1-3: Two fusion protein containing the HIS Tag, Primary antibody: P00002(6×His Tag Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 3 seconds |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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