别名:ACAD-11应用:WB,IHC
反应种属:Human
规格:50μl/100μl
Description |
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The deduced full-length ACAD11 protein contains an N-terminal aminoglycoside phosphotransferase domain, followed by a mitochondria localization signal and ACAD N-terminal, middle, and C-terminal domains. It also has a glycosylation site and a C-terminal peroxisome-targeting signal. The ACAD domain contains a catalytic aspartate rather than the catalytic glutamate found in most other ACADs. Real-time RT-PCR detected variable ACAD11 expression in all tissues examined, with highest expression in adult brain, followed by heart, liver, spinal cord, and kidney. Western blot analysis of fractionated human cerebellum and kidney revealed that ACAD11 associated with mitochondrial membranes, but not mitochondrial matrix. Immunohistochemical analysis showed that ACAD11 colocalized with a mitochondrial marker in human neuroblastoma cells. |
Specification | |
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Aliases | ACAD-11 |
Entrez GeneID | 84129; |
Swissprot | Q709F0 |
WB Predicted band size | 87kDa |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Fusion protein corresponding to a region derived from internal residues of human Acyl-CoA dehydrogenase family, member 11 |
Formulation | Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol. |
Application | |
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WB | 1/200-1/1000 |
IHC | 115-1/50 |
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Gel: 8%SDS-PAGE Lysates (from left to right): Human fetal liver tissue Amount of lysate: 40ug per lane Primary antibody: 1/300 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute |
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Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using P42793 at dilution 1/15. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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