Rabbit Polyclonal Antibody to ACF

包装: 1G|100G|500G|1KG|100KG

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别名:APOBEC1 complementation factor, APOBEC1-stimulating protein, A1CF, ACF, ASP {ECO:0000312|EMBL:CAB947541}
应用:WB,IHC,FCM
反应种属:Human
规格：50μl/100μl

Description
Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events.

Description

Mammalian apolipoprotein B mRNA undergoes site-specific C
to U deamination, which is mediated by a multi-component enzyme
complex containing a minimal core composed of APOBEC-1 and a
complementation factor encoded by this gene. The gene product has
three non-identical RNA recognition motifs and belongs to the hnRNP
R family of RNA-binding proteins. It has been proposed that this
complementation factor functions as an RNA-binding subunit and
docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest
that the protein may also be involved in other RNA editing or RNA
processing events.

Specification
Aliases	APOBEC1 complementation factor, APOBEC1-stimulating protein, A1CF, ACF, ASP {ECO:0000312\|EMBL:CAB947541}
Entrez GeneID	29974
Swissprot	Q9NQ94
WB Predicted band size	65.2kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This ACF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-423 amino acids from the C-terminal region of human ACF.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
FCM	1/10-1/50

	ACF Antibody (C-term) (Cat. #P30826) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the ACF antibody detected the ACF protein (arrow).
	ACF Antibody (C-term) (Cat. #P30826) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	ACF Antibody (C-term) (Cat. #P30826) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACF Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.