中文 
别名:Activated CDC42 kinase 1; ACK-1; EC=2.7.10.2; EC=2.7.11.1; Tyrosine kinase non-receptor protein 2应用:WB
反应种属:Human,Mouse
规格：50μl/100μl
| Description |
|---|
| Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on ‘Tyr-176’. Phosphorylates AR on ‘Tyr-267’ and ‘Tyr-363’ thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on ‘Tyr-287’. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR.Manser E., Nature 363:364-367(1993) [PubMed: 8497321].Ota T.,Nat. Genet. 36:40-45(2004) [PubMed: 14702039].Muzny D.M., Nature 440:1194-1198(2006) [PubMed: 16641997]. |
| Specification | |
|---|---|
| Aliases | Activated CDC42 kinase 1; ACK-1; EC=2.7.10.2; EC=2.7.11.1; Tyrosine kinase non-receptor protein 2 |
| Entrez GeneID | 10188; |
| Swissprot | Q07912 |
| WB Predicted band size | 115kDa |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from Internal of human ACK1. |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Application | |
|---|---|
| WB | 1/500-1/3000 |
 |
Western blot analysis of extracts from 293 cells (Lane 2) and K562 cells (Lane 3), using ACK1 (Ab-284) antiobdy P41654. The lane on the left is treated with synthesized peptide. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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