Rabbit Polyclonal Antibody to ACK1 (Phospho-Tyr284)

包装: 1G|100G|500G|1KG|100KG

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别名:ACK1; Activated p21cdc42Hs kinase; EC 2.7.10.2; kinase ACK1;
应用:WB,IHC,ICC
反应种属:Human,Mouse
规格：50μl/100μl

Description
Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Manser E., Nature 363:364-367(1993)Ota T., Nat. Genet. 36:40-45(2004)Eisenmann K.M., Nat. Cell Biol. 1:507-513(1999)

Description

Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Manser E., Nature 363:364-367(1993)Ota T., Nat. Genet. 36:40-45(2004)Eisenmann K.M., Nat. Cell Biol. 1:507-513(1999)

Specification
Aliases	ACK1; Activated p21cdc42Hs kinase; EC 2.7.10.2; kinase ACK1;
Entrez GeneID	10188;
Swissprot	Q07912
WB Predicted band size	120kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse
Immunogen	Peptide sequence around phosphorylation site of tyrosine 284 (D-H-Y(p)-V-M) derived from Human ACK1.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/100-1/200

	Western blot analysis of extracts from HepG2 cells treated with EGF using ACK1 (Phospho-Tyr284) Antibody P40385.The lane on the right is treated with the antigen-specific peptide.
	Immunofluorescence staining of methanol-fixed A549 cells using ACK1 (Phospho-Tyr284) Antibody P40385.
	Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ACK1 (Phospho-Tyr284) antibody P40385 (left)or the same antibody preincubated with blocking peptide (right).