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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to ACPP

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别名:Prostatic acid phosphatase, PAP, 5′-nucleotidase, 5′-NT, Ecto-5′-nucleotidase, Thiamine monophosphatase, TMPase, PAPf39, ACPP
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
ACPP is an enzyme which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland.
Specification
Aliases Prostatic acid phosphatase, PAP, 5′-nucleotidase, 5′-NT, Ecto-5′-nucleotidase, Thiamine monophosphatase, TMPase, PAPf39, ACPP
Entrez GeneID 55
Swissprot P15309
WB Predicted band size 44.6kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This ACPP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 313-341 amino acids from the C-terminal region of human ACPP.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application
WB 1/500-1/1000
IHC 1/100-1/500

All lanes : Anti-ACPP Antibody (C-term) at 1:1000 dilution
Lane 1: Mouse prostate whole tissue lysate
Lane 2: Rat prostate whole tissue lysate
Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 45 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-ACPP Antibody (C-term) at 1:500 dilution
Lane 1: PC-3 whole cell lysate
Lane 2: DU145 whole cell lysate
Lane 3: U-251MG whole cell lysate
Lane 4: Mouse lung whole tissue lysate
Lane 5: Mouse prostate whole tissue lysate
Lane 5: Rat prostate whole tissue lysate
Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 45 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ACPP antibody (C-term) (Cat.#P34825), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。