Rabbit Polyclonal Antibody to ACTA1/Alpha-actin

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别名:Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA
应用:WB,IHC,ICC,FCM
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

Description

The product encoded by this gene belongs to the actin
family of proteins, which are highly conserved proteins that play a
role in cell motility, structure and integrity. Alpha, beta and
gamma actin isoforms have been identified, with alpha actins being
a major constituent of the contractile apparatus, while beta and
gamma actins are involved in the regulation of cell motility. This
actin is an alpha actin that is found in skeletal muscle. Mutations
in this gene cause nemaline myopathy type 3, congenital myopathy
with excess of thin myofilaments, congenital myopathy with cores,
and congenital myopathy with fiber-type disproportion, diseases
that lead to muscle fiber defects.

Specification
Aliases	Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA
Entrez GeneID	58
Swissprot	P68133
WB Predicted band size	42.1kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This ACTA1/Alpha-actin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from the C-terminal region of human ACTA1/Alpha-actin.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
ICC	1/10-1/50
FCM	1/10-1/50

	ACTA1/α-actin Antibody (C-term) (Cat. #P31531) western blot analysis in A549,RD cell line and human placenta lysates (35ug/lane).This demonstrates the ACTA1/α-actin antibody detected the ACTA1/α-actin protein (arrow).
	Confocal immunofluorescent analysis of ACTA1/α-actin Antibody (C-term)(Cat#P31531) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
	ACTA1/?-actin Antibody (C-term) (Cat. #P31531) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	ACTA1/?-actin Antibody (C-term) (P31531)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1/?-actin Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.