Rabbit Polyclonal Antibody to Actin

包装: 1G|100G|500G|1KG|100KG

现货支持定制

QQ咨询
| 微信咨询

满200元免运费
正品保障提供发票
现货闪电当时发货

别名:ACTB; Actin; cytoplasmic 1; Beta-actin; POTEKP; ACTBL3; FKSG30; Putative beta-actin-like protein 3; Kappa-actin; POTE ankyrin domain family member K; ACTG1; ACTB; ACTG; Actin; cytoplasmic 2; Gamma-actin
应用:WB,IHC
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.

Description

beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.

Specification
Aliases	ACTB; Actin; cytoplasmic 1; Beta-actin; POTEKP; ACTBL3; FKSG30; Putative beta-actin-like protein 3; Kappa-actin; POTE ankyrin domain family member K; ACTG1; ACTB; ACTG; Actin; cytoplasmic 2; Gamma-actin
Entrez GeneID	60/71
Swissprot	P60709/P63261
WB Predicted band size	Calculated MW: 42 kDa; Observed MW: 45 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	The antiserum was produced against synthesized peptide derived from human Actin. AA range:326-375
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Application
WB	1/500-1/1000
IHC	1/50-1/100
ELISA	1/10000

	Western blot analysis of Actin in various lysates using Actin antibody.
	Western blot analysis of Actin in HeLa lysates using Actin antibody.
	Western blot analysis of Actin in various lysates using Actin antibody.
	Immunohistochemistry analysis of paraffin-embedded Human skeletal muscle using Actin antibody.High-pressure and temperature Tris-EDTA pH 8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody. was preabsorbed by immunogen peptide.