中文 
别名:Adenosine deaminase, Adenosine aminohydrolase, ADA, ADA1应用:WB,FCM
反应种属:Human, Mouse
规格：50μl/100μl
| Description |
|---|
| This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. |
| Specification | |
|---|---|
| Aliases | Adenosine deaminase, Adenosine aminohydrolase, ADA, ADA1 |
| Entrez GeneID | 100 |
| Swissprot | P00813 |
| WB Predicted band size | 40.8kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This ADA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-314 amino acids from the C-terminal region of human ADA. |
| Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Application | |
|---|---|
| WB | 1/1000 |
| FCM | 1/10-1/50 |
 |
All lanes : Anti-ADA Antibody (C-term) at 1:1000 dilution Lane 1: MOLT-4 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
 |
Overlay histogram showing Jurkat cells stained with P34588(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
暂无评论
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
发表回复