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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to ADAMTS17

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别名:A disintegrin and metalloproteinase with thrombospondin motifs 17, ADAM-TS 17, ADAM-TS17, ADAMTS-17, 3424-, ADAMTS17
应用:WB,IHC
反应种属:Human
规格:50μl/100μl

Specification
Aliases A disintegrin and metalloproteinase with thrombospondin motifs 17, ADAM-TS 17, ADAM-TS17, ADAMTS-17, 3424-, ADAMTS17
Entrez GeneID 170691
Swissprot Q8TE56
WB Predicted band size 121.1kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This ADAMTS17 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 508-540 amino acids from the Central region of human ADAMTS17.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/500-1/2000
IHC 1/100

All lanes : Anti-ADAMTS17 Antibody (Center) at 1:500 dilution
Lane 1: A431 whole cell lysate
Lane 2: 293 whole cell lysate
Lane 3: SK-BR-3 whole cell lysate
Lane 4: RPMI 8226 whole cell lysate
Lane 5: MOLT-4 whole cell lysate
Lane 6: PC-3 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 121 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-ADAMTS17 Antibody (Center) at 1:500 dilution
Lane 1: A431 whole cell lysate
Lane 2: 293 whole cell lysate
Lane 3: SK-BR-3 whole cell lysate
Lane 4: RPMI 8226 whole cell lysate
Lane 5: PC-3 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 121 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-ADAMTS17 Antibody (Center) at 1:2000 dilution + Molt-4 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 121 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of P34793 on paraffin-embedded Human brain tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of P34793 on paraffin-embedded Human lung tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of P34793 on paraffin-embedded Human lung tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of P34793 on paraffin-embedded Human brain tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。