Rabbit Polyclonal Antibody to AKT

包装: 1G|100G|500G|1KG|100KG

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别名:MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
应用:WB,IHC,ICC,FCM,IP
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.

Description

AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.

Specification
Aliases	MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
Entrez GeneID	10000
Swissprot	Q9Y243
WB Predicted band size	Calculated MW: 56 kDa; Observed MW: 56 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Recombinant protein of human AKT3
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Application
IP	1/20
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200
FCM	1/50-1/100

	Western blot analysis of AKT in 3T3, C6 lysates using AKT antibody.
	Western blot analysis of Akt (pan) in Jurkat lysates using Akt antibody
	Immunocytochemistry analysis of AKT (green) in A549 using AKT antibody,and DAPI(blue).
	Immunohistochemistry analysis of paraffin-embedded Human tonsil using AKT1/2/3 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.