Rabbit Polyclonal Antibody to AKT

包装: 1G|100G|500G|1KG|100KG

现货支持定制

QQ咨询
| 微信咨询

400-880-2824

满200元免运费
正品保障提供发票
现货闪电当时发货

别名:AKT1
应用:WB,IHC,ICC,FCM,IP
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.

Description

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.

Specification
Aliases	AKT1
Entrez GeneID	207/208/10000
Swissprot	P31749/P31751/Q9Y243
WB Predicted band size	Calculated MW: 56 kDa; Observed MW: 56 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	A synthesized peptide derived from human AKT1/2/3
Formulation	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
IP	1/20
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200
FCM	1/50-1/100

	Western blot analysis of AKT in K562, A549 and Jurkat lysates using AKT antibody.
	Immunofluorescence analysis of AKT in MCF-7 using AKT antibody.
	Immunohistochemistry analysis of paraffin-embedded Human testis using AKT antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.