Rabbit Polyclonal Antibody to AKT1/3 (Phospho-Tyr437/434)

包装: 1G|100G|500G|1KG|100KG

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别名:EC 2.7.11.1; RAC-PK-alpha; Protein kinase B; PKB; C-AKT
应用:WB,IHC
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at ‘Ser-50’ negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling.

Description

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at ‘Ser-50’ negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling.

Specification
Aliases	EC 2.7.11.1; RAC-PK-alpha; Protein kinase B; PKB; C-AKT
Entrez GeneID	207;
Swissprot	P31749
WB Predicted band size	56kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Peptide sequence around phosphorylation site of tyrosine 437 (T-R-Y(p)-F-D) derived from Human AKT1/3.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100

	Western blot analysis of extracts from K562 cells, treated with insulin (0.01U/ml, 15mins), using AKT1/3 (Phospho-Tyr437/434) antibody P40721. The lane on the right is treated with the synthesized peptide.
	Immunohistochemistry analysis of paraffin-embedded human brain tissue using AKT1/3 (Phospho-Tyr437/434) antibody P40721. The picture on the right is treated with the synthesized peptide.