Rabbit Polyclonal Antibody to Alkaline Phosphatase

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别名:ALPL; Alkaline phosphatase; tissue-nonspecific isozyme; AP-TNAP; TNSALP; Alkaline phosphatase liver/bone/kidney isozyme
应用:WB,IHC,ICC,FCM,IP
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme.

Description

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme.

Specification
Aliases	ALPL; Alkaline phosphatase; tissue-nonspecific isozyme; AP-TNAP; TNSALP; Alkaline phosphatase liver/bone/kidney isozyme
Entrez GeneID	249
Swissprot	P05186
WB Predicted band size	Calculated MW: 57 kDa; Observed MW: 75 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	A synthesized peptide derived from human Alkaline Phosphatase
Formulation	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
IP	1/20
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200
FCM	1/50-1/100

	Western blot analysis of Alkaline Phosphatase in (1)HepG2 lysates; (2)JAR lysates using Alkaline Phosphatase antibody.
	Western blot analysis of Alkaline Phosphatase in SKOV-3, Jurkat and MCF-7 lysates using Alkaline Phosphatase antibody.
	Immunofluorescence analysis of Alkaline Phosphatase in Hela using Alkaline Phosphatase antibody.
	Immunohistochemistry analysis of paraffin-embedded Human liver using Alkaline Phosphatase antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.