Rabbit Polyclonal Antibody to AML1 (Ab-435)

包装: 1G|100G|500G|1KG|100KG

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别名:Acute myeloid leukemia 1 protein; CBF-alpha 2; CBFA2; Core-binding factor; alpha 2 subunit
应用:WB
反应种属:Human
规格：50μl/100μl

Description
CBF binds to the core site, 5′-PYGPYGGT-3′, of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B-dependent transcriptional activation.Miyoshi H., Proc. Natl. Acad. Sci. U.S.A. 88:10431-10434(1991).Sacchi N., Genes Chromosomes Cancer 11:226-236(1994).Nucifora G., Blood 81:2728-2734(1993).

Description

CBF binds to the core site, 5′-PYGPYGGT-3′, of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B-dependent transcriptional activation.Miyoshi H., Proc. Natl. Acad. Sci. U.S.A. 88:10431-10434(1991).Sacchi N., Genes Chromosomes Cancer 11:226-236(1994).Nucifora G., Blood 81:2728-2734(1993).

Specification
Aliases	Acute myeloid leukemia 1 protein; CBF-alpha 2; CBFA2; Core-binding factor; alpha 2 subunit
Entrez GeneID	861;
Swissprot	Q01196
WB Predicted band size	53kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Synthesized non-phosphopeptide derived from human AML1 around the phosphorylation site of serine 435 (S-N-S(p)-P-T).
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000

	Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using AML1 (Ab-435) antibody P41742.
	Western blot analysis of extracts from 293 cells (Lane 2) and Hela cells (Lane 3), using AML1 (Ab-435) antiobdy P41742. The lane on the left is treated with synthesized peptide.