别名:Alpha-amylase 2B,4-alpha-D-glucan glucanohydrolase 2B, Carcinoid alpha-amylase, AMY2B应用:WB,IHC,ICC,FCM
反应种属:Human
规格:50μl/100μl
| Description |
|---|
| Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. |
| Specification | |
|---|---|
| Aliases | Alpha-amylase 2B,4-alpha-D-glucan glucanohydrolase 2B, Carcinoid alpha-amylase, AMY2B |
| Entrez GeneID | 280 |
| Swissprot | P19961 |
| WB Predicted band size | 57.7kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This AMY2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-100 amino acids from the N-terminal region of human AMY2B. |
| Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Application | |
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| ICC | 1/10-1/50 |
| FCM | 1/10-1/50 |
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AMY2B Antibody (N-term) (Cat. #P30831) western blot analysis in HL-60,CEM and K562 cell line lysates (35ug/lane).This demonstrates the AMY2B antibody detected the AMY2B protein (arrow). |
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Confocal immunofluorescent analysis of AMY2B Antibody (N-term) (Cat#P30831) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
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AMY2B Antibody (N-term) (Cat. #P30831) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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AMY2B antibody (N-term) (Cat. #P30831) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AMY2B antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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