Rabbit Polyclonal Antibody to ANAPC5

包装: 1G|100G|500G|1KG|100KG

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别名:Anaphase-promoting complex subunit 5, APC5, Cyclosome subunit 5, ANAPC5, APC5
应用:WB,FCM
反应种属:Human
规格：50μl/100μl

Description
This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.

Description

This gene encodes a tetratricopeptide repeat-containing
component of the anaphase promoting complex/cyclosome (APC/C), a
large E3 ubiquitin ligase that controls cell cycle progression by
targeting a number of cell cycle regulators such as B-type cyclins
for 26S proteasome-mediated degradation through ubiquitination. The
encoded protein is required for the proper ubiquitination function
of APC/C and for the interaction of APC/C with transcription
coactivators. It also interacts with polyA binding protein and
represses internal ribosome entry site-mediated translation.
Multiple transcript variants encoding different isoforms have been
found for this gene. These differences cause translation initiation
at a downstream AUG and result in a shorter protein (isoform b),
compared to isoform a.

Specification
Aliases	Anaphase-promoting complex subunit 5, APC5, Cyclosome subunit 5, ANAPC5, APC5
Entrez GeneID	51433
Swissprot	Q9UJX4
WB Predicted band size	85.1kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This ANAPC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the Central region of human ANAPC5.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
FCM	1/10-1/50

	ANAPC5 Antibody (Center) (Cat. #P30883) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ANAPC5 antibody detected the ANAPC5 protein (arrow).
	ANAPC5 Antibody (Center) (Cat. #P30883) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.