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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to ANKH

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别名:Progressive ankylosis protein homolog, ANK, ANKH, KIAA1581
应用:WB,IHC,FCM
反应种属:Human, Mouse
规格:50μl/100μl

Description
ANKH is a multipass transmembrane protein that is expressed in joints and other tissues and controls pyrophosphate levels in cultured cells. Progressive ankylosis-mediated control of pyrophosphate levels has been suggested as a possible mechanism regulating tissue calcification and susceptibility to arthritis in higher animals.
Specification
Aliases Progressive ankylosis protein homolog, ANK, ANKH, KIAA1581
Entrez GeneID 56172
Swissprot Q9HCJ1
WB Predicted band size 54.2kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This ANKH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 464-492 amino acids from the C-terminal region of human ANKH.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000
IHC 1/100-1/500
FCM 1/10-1/50

Anti-ANKH Antibody (C-term) at 1:1000 dilution + U-2OS whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 54 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of ANKH Antibody (C-term) (Cat. #P33881) in mouse cerebellum tissue lysates (35ug/lane). ANKH (arrow) was detected using the purified Pab.
ANKH Antibody (C-term) (Cat. #P33881) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ANKH Antibody (C-term) (Cat. #P33881) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ANKH Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。