别名:Progressive ankylosis protein homolog, ANK, ANKH, KIAA1581应用:WB,IHC,FCM
反应种属:Human, Mouse
规格:50μl/100μl
| Description |
|---|
| ANKH is a multipass transmembrane protein that is expressed in joints and other tissues and controls pyrophosphate levels in cultured cells. Progressive ankylosis-mediated control of pyrophosphate levels has been suggested as a possible mechanism regulating tissue calcification and susceptibility to arthritis in higher animals. |
| Specification | |
|---|---|
| Aliases | Progressive ankylosis protein homolog, ANK, ANKH, KIAA1581 |
| Entrez GeneID | 56172 |
| Swissprot | Q9HCJ1 |
| WB Predicted band size | 54.2kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This ANKH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 464-492 amino acids from the C-terminal region of human ANKH. |
| Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Application | |
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| FCM | 1/10-1/50 |
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Anti-ANKH Antibody (C-term) at 1:1000 dilution + U-2OS whole cell lysate
Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Western blot analysis of ANKH Antibody (C-term) (Cat. #P33881) in mouse cerebellum tissue lysates (35ug/lane). ANKH (arrow) was detected using the purified Pab. |
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ANKH Antibody (C-term) (Cat. #P33881) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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ANKH Antibody (C-term) (Cat. #P33881) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ANKH Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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