Rabbit Polyclonal Antibody to APEX1

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别名:APE; APX; APE1; APEN; APEX; HAP1; REF1
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5′ to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.

Description

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5′ to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.

Specification
Aliases	APE; APX; APE1; APEN; APEX; HAP1; REF1
Swissprot	P27695
WB Predicted band size	36 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	Fusion protein of human APEX1
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/500-1/2000
IHC	1/15-1/50
ELISA	1/2000-1/5000

	Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-6: Hela cells, human liver cancer tissue, Raji cells, Jurkat cells, 293T cells, PC3 cells, Primary antibody: P07075(APEX1 Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
	The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using P07075(APEX1 Antibody) at dilution 1/20. (Original magnification: ×200)
	The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using P07075(APEX1 Antibody) at dilution 1/20. (Original magnification: ×200)