Rabbit Polyclonal Antibody to APG10L (C-term S116)

包装: 1G|100G|500G|1KG|100KG

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别名:Ubiquitin-like-conjugating enzyme ATG10, 632-, Autophagy-related protein 10, APG10-like, ATG10, APG10L
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).

Description

Autophagy is a process for the bulk degradation of
cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme
involved in 2 ubiquitin-like modifications essential for
autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261)
conjugation and modification of a soluble form of MAP-LC3
(MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a
membrane-bound form (Nemoto et al., 2003 [PubMed
12890687]).

Specification
Aliases	Ubiquitin-like-conjugating enzyme ATG10, 632-, Autophagy-related protein 10, APG10-like, ATG10, APG10L
Entrez GeneID	83734
Swissprot	Q9H0Y0
WB Predicted band size	25.3kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This APG10L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-121 amino acids of human APG10L.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500

	APG10L Antibody (C-term S116) (Cat. #P31444) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the APG10L antibody detected the APG10L protein (arrow).
	Western blot analysis of APG10L (arrow) using rabbit polyclonal APG10L Antibody (C-term S116) (Cat. #P31444). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG10L gene.
	APG10L Antibody (C-term S116) (Cat. #P31444)immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ANKS1B APG10L Antibody (C-term S116) for immunohistochemistry. Clinical relevance has not been evaluated.