Rabbit Polyclonal Antibody to APLF (Ab-116)

包装: 1G|100G|500G|1KG|100KG

现货支持定制

QQ咨询
| 微信咨询

400-880-2824

满200元免运费
正品保障提供发票
现货闪电当时发货

别名:APFL; aprataxin and PNK-like factor; C2orf13; Xip1;
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3′-5′ exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage.Saunders W.S., J. Cell Sci. 104:573-582(1993).Ye Q., J. Biol. Chem. 271:14653-14656(1996).Ye Q., J. Biol. Chem. 272:14983-14989(1997).

Description

Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3′-5′ exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage.Saunders W.S., J. Cell Sci. 104:573-582(1993).Ye Q., J. Biol. Chem. 271:14653-14656(1996).Ye Q., J. Biol. Chem. 272:14983-14989(1997).

Specification
Aliases	APFL; aprataxin and PNK-like factor; C2orf13; Xip1;
Entrez GeneID	200558;
Swissprot	Q8IW19
WB Predicted band size	57kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Synthesized non-phosphopeptide derived from human APLF around the phosphorylation site of serine 116 (R-N-S(p)-Q-V).
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100

	Western blot analysis of extracts from HT-29 cells, using APLF (Ab-116) antibody P41744.
	Western blot analysis of extracts from COS7 cells (Lane 2), using APLF (Ab-116) antiobdy P41744. The lane on the left is treated with systhesized peptide.
	Immunohistochemistry analysis of paraffin-embedded human brain tissue using APLF (Ab-116) antibody P41744.