库存-现货
瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to AQP5

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别名:Aquaporin-5, AQP-5, AQP5
应用:WB,FCM
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
Aquaporin 5 (AQP5) is a water channel protein. Aquaporins
are a family of small integral membrane proteins related to the
major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in
the generation of saliva, tears and pulmonary secretions. AQP0,
AQP2, AQP5, and AQP6 are closely related and all map to 12q13.
Specification
Aliases Aquaporin-5, AQP-5, AQP5
Entrez GeneID 362
Swissprot P55064
WB Predicted band size 28.3kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This AQP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-256 amino acids from the C-terminal region of human AQP5.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/2000
FCM 1/25

Anti-AQP5 Antibody (C-term) at 1:2000 dilution + Jurkat whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 28 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing U-2 OS cells stained with P34518 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34518, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。