Rabbit Polyclonal Antibody to ARFGAP1

包装: 1G|100G|500G|1KG|100KG

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别名:ARF1GAP; HRIHFB2281
应用:WB,IHC
反应种属:Human, Mouse
规格：50μl/100μl

Description
The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene.

Description

The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene.

Specification
Aliases	ARF1GAP; HRIHFB2281
Swissprot	Q8N6T3
WB Predicted band size	45 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	Fusion protein of human ARFGAP1
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/1000-1/5000
IHC	1/25-1/100
ELISA	1/2000-1/10000

	Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-4: 293T cells, A549 cells, PC3 cells, NIH/3T3 cells, Primary antibody: P07878(ARFGAP1 Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
	The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using P07878(ARFGAP1 Antibody) at dilution 1/30. (Original magnification: ×200)