别名:Ecto-ADP-ribosyltransferase 3, ADP-ribosyltransferase C2 and C3 toxin-like 3, ARTC3, Mono(ADP-ribosyl)transferase 3, NAD(P)(+)–arginine ADP-ribosyltransferase 3, ART3, TMART应用:WB
反应种属:Human
规格:50μl/100μl
Description |
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Mono-ADP-ribosylation involves the transfer of the ADP-ribose moiety from NAD+ to a specific amino acid in the target protein. The rodent mono-ADP-ribosyltransferase RT6 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein specifically expressed at the cell surface of rat and mouse T lymphocytes. The predicted 367-amino acid human ART3 protein has an estimated molecular mass of 41.5 kD and contains a hydrophobic peptide signal at its N terminus, 3 consensus motifs specific to enzymes catalyzing ADP-ribose transfer, a hydrophobic C-terminal sequence characteristic of a GPI-anchored protein, a novel motif repeated 3 times at its C terminus, and 1 potential glycosylation site.1 The ART3 and rodent RT6 proteins share 35% amino acid identity. |
Specification | |
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Aliases | Ecto-ADP-ribosyltransferase 3, ADP-ribosyltransferase C2 and C3 toxin-like 3, ARTC3, Mono(ADP-ribosyl)transferase 3, NAD(P)(+)–arginine ADP-ribosyltransferase 3, ART3, TMART |
Entrez GeneID | 419 |
Swissprot | Q13508 |
WB Predicted band size | 43.9kDa |
Host/Isotype | Rabbit IgG |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This ART3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 280-310 amino acids from the C-terminal region of human ART3. |
Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
Application | |
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WB | 1/1000-1/4000 |
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Anti-ART3 Antibody at 1:4000 dilution + human testis lysate
Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Western blot analysis of ART3 Antibody (C-term) in HL60 cell line lysates (25ug/lane). ART3(arrow) was detected using the purified Pab (4 ug/ml). |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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