库存-现货
瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to ASMT

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别名:Acetylserotonin O-methyltransferase, Hydroxyindole O-methyltransferase, HIOMT, ASMT
应用:WB,IHC,FCM
反应种属:Human
规格:50μl/100μl

Description
ASMT belongs to the methyltransferase superfamily, and is located in the pseudoautosomal region (PAR) at the end of the short arms of the X and Y chromosomes. The encoded enzyme catalyzes the final reaction in the synthesis of melatonin, and is abundant in the pineal gland.
Specification
Aliases Acetylserotonin O-methyltransferase, Hydroxyindole O-methyltransferase, HIOMT, ASMT
Entrez GeneID 438
Swissprot P46597
WB Predicted band size 38.5kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This ASMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-241 amino acids from the Central region of human ASMT.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/500-1/1000
IHC 1/100-1/500
FCM 1/10-1/50

Western blot analysis of lysate from Y79 cell line, using ASMT Antibody (Center)(Cat. #P32860). P32860 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Western blot analysis of ASMT Antibody (Center) (Cat. #P32860) in 293 cell line lysates (35ug/lane). ASMT (arrow) was detected using the purified Pab.
ASMT Antibody (Center) (Cat. #P32860) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ASMT Antibody (Center) (Cat. #P32860) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ASMT Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。