Rabbit Polyclonal Antibody to ATF-2 (Ab-472)

包装: 1G|100G|500G|1KG|100KG

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别名:Activating 2; ATF2; cAMP response element binding protein CRE- BP1; CREB2; CREBP1
应用:WB
反应种属:Human
规格：50μl/100μl

Description
Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5′-TGACGTCA-3′) or to AP-1 (activator protein 1) consensus sequences (5′-TGACTCA-3′). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro. In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.Maekawa T., EMBO J. 8:2023-2028(1989).Yang L., J. Immunol. 158:2522-2525(1997).Hillier L.W., Nature 434:724-731(2005).

Description

Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5′-TGACGTCA-3′) or to AP-1 (activator protein 1) consensus sequences (5′-TGACTCA-3′). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro. In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.Maekawa T., EMBO J. 8:2023-2028(1989).Yang L., J. Immunol. 158:2522-2525(1997).Hillier L.W., Nature 434:724-731(2005).

Specification
Aliases	Activating 2; ATF2; cAMP response element binding protein CRE- BP1; CREB2; CREBP1
Entrez GeneID	1386;
Swissprot	P15336
WB Predicted band size	70kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Synthesized non-phosphopeptide derived from human ATF-2 around the phosphorylation site of serine 472 (A-L-S(p)-Q-I).
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000

	Western blot analysis of extracts from HeLa cells and 293 cells, using ATF-2 (Ab-472) antibody P41746.
	Western blot analysis of extracts from JK cells (Lane 2), using ATF-2 (Ab-472) antiobdy P41746. The lane on the left is treated with synthesized peptide