Rabbit Polyclonal Antibody to ATG4B

包装: 1G|100G|500G|1KG|100KG

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别名:Cysteine protease ATG4B; EC 3.4.22.-; Autophagy-related protein 4 homolog B; hAPG4B; Autophagin-1
应用:WB,IHC
反应种属:Human,Mouse
规格：50μl/100μl

Description
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.Kabeya Y., J. Cell Sci. 117:2805-2812(2004).Marino G., J. Biol. Chem. 278:3671-3678(2003).Ota T.,Nat. Genet. 36:40-45(2004).

Description

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.Kabeya Y., J. Cell Sci. 117:2805-2812(2004).Marino G., J. Biol. Chem. 278:3671-3678(2003).Ota T.,Nat. Genet. 36:40-45(2004).

Specification
Aliases	Cysteine protease ATG4B; EC 3.4.22.-; Autophagy-related protein 4 homolog B; hAPG4B; Autophagin-1
Entrez GeneID	23192;
Swissprot	Q9Y4P1
WB Predicted band size	44kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse
Immunogen	Synthesized peptide derived from internal of human ATG4B.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100

	Western blot analysis of extracts from Jurkat cells, using ATG4B antibody P42150.
	Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using ATG4B antibody P42150.