中文 
别名:Sodium/potassium-transporting ATPase subunit alpha-4, Na(+)/K(+) ATPase alpha-4 subunit, 3.6.3.9, Sodium pump subunit alpha-4, ATP1A4, ATP1AL2应用:WB,FCM
反应种属:Human
规格：50μl/100μl
| Description |
|---|
| This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Plays a role in sperm motility. |
| Specification | |
|---|---|
| Aliases | Sodium/potassium-transporting ATPase subunit alpha-4, Na(+)/K(+) ATPase alpha-4 subunit, 3.6.3.9, Sodium pump subunit alpha-4, ATP1A4, ATP1AL2 |
| Entrez GeneID | 480 |
| Swissprot | Q13733 |
| WB Predicted band size | 114.2kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ATP1A4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 12-46 amino acids from the human region of human ATP1A4. |
| Application | |
|---|---|
| WB | 1/2000 |
| FCM | 1/25 |
 |
Anti-ATP1A4 Antibody (N-Term) at 1:2000 dilution + Human brain lysate
Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 114 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
 |
Overlay histogram showing HeLa cells stained with P34484(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34484, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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