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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to ATP2A3 (N-Term)

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别名:Sarcoplasmic/endoplasmic reticulum calcium ATPase 3, SERCA3, SR Ca(2+)-ATPase 3, Calcium pump 3, ATP2A3
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
Specification
Aliases Sarcoplasmic/endoplasmic reticulum calcium ATPase 3, SERCA3, SR Ca(2+)-ATPase 3, Calcium pump 3, ATP2A3
Entrez GeneID 489
Swissprot Q93084
WB Predicted band size 109.3kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This ATP2A3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 13-47 amino acids from human ATP2A3.
Application
WB 1/2000
IHC 1/100-1/500

All lanes : Anti-ATP2A3 Antibody (N-Term) at 1:2000 dilution
Lane 1: HL-60 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: K562 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 114 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

P33355 staining ATP2A3 in human brain tissue sections by Immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。