Rabbit Polyclonal Antibody to ATP6V0B

包装: 1G|100G|500G|1KG|100KG

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别名:V-type proton ATPase 21 kDa proteolipid subunit, V-ATPase 21 kDa proteolipid subunit, Vacuolar proton pump 21 kDa proteolipid subunit, hATPL, ATP6V0B, ATP6F
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c’, c”, and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is part of the transmembrane V0 domain and is the human counterpart of yeast VMA16. Two alternatively spliced transcript variants that encode different proteins have been found for this gene.

Description

This gene encodes a component of vacuolar ATPase
(V-ATPase), a multisubunit enzyme that mediates acidification of
eukaryotic intracellular organelles. V-ATPase dependent organelle
acidification is necessary for such intracellular processes as
protein sorting, zymogen activation, receptor-mediated endocytosis,
and synaptic vesicle proton gradient generation. V-ATPase is
composed of a cytosolic V1 domain and a transmembrane V0 domain.
The V1 domain consists of three A and three B subunits, two G
subunits plus the C, D, E, F, and H subunits. The V1 domain
contains the ATP catalytic site. The V0 domain consists of five
different subunits: a, c, c’, c”, and d. Additional isoforms of
many of the V1 and V0 subunit proteins are encoded by multiple
genes or alternatively spliced transcript variants. This encoded
protein is part of the transmembrane V0 domain and is the human
counterpart of yeast VMA16. Two alternatively spliced transcript
variants that encode different proteins have been found for this
gene.

Specification
Aliases	V-type proton ATPase 21 kDa proteolipid subunit, V-ATPase 21 kDa proteolipid subunit, Vacuolar proton pump 21 kDa proteolipid subunit, hATPL, ATP6V0B, ATP6F
Entrez GeneID	533
Swissprot	Q99437
WB Predicted band size	21.4kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This ATP6V0B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-131 amino acids from the Central region of human ATP6V0B.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500

	ATP6V0B Antibody (Center) (Cat. #P30768) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the EKI2 antibody detected the EKI2 protein (arrow).
	ATP6V0B Antibody (Center) (Cat. #P30768) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP6V0B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.