Rabbit Polyclonal Antibody to B-RAF (Ab-446)

包装: 1G|100G|500G|1KG|100KG

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别名:B-RAF; B-RAF proto-oncogene serine/threonine-protein kinase; B-Raf proto-oncogene serine/threonine-protein kinase; BRAF; BRAF1
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.Stephens R.M., Mol. Cell. Biol. 12:3733-3742(1992).Albert S., Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.Hillier L.W., Nature 424:157-164(2003)

Description

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.Stephens R.M., Mol. Cell. Biol. 12:3733-3742(1992).Albert S., Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.Hillier L.W., Nature 424:157-164(2003)

Specification
Aliases	B-RAF; B-RAF proto-oncogene serine/threonine-protein kinase; B-Raf proto-oncogene serine/threonine-protein kinase; BRAF; BRAF1
Entrez GeneID	673;
Swissprot	P15056
WB Predicted band size	84kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Synthesized non-phosphopeptide derived from human B-RAF around the phosphorylation site of serine 446 (R-D-S(p)-S-D).
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100

	Western blot analysis of extracts from 293 cells, treated with insulin (0.01U/ml, 15mins), using B-RAF (Ab-446) antibody P41706.
	Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using B-RAF (Ab-446) antibody P41706.