Rabbit Polyclonal Antibody to BAF250B

包装: 1G|100G|500G|1KG|100KG

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别名:ARID domain-containing protein 1B; Osa homolog 2; hOsa2; p250R; BRG1-binding protein hELD/OSA1
应用:WB,ICC
反应种属:Human,Rat
规格：50μl/100μl

Description
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth By similarity. Binds DNA non-specifically.Mungall A.J., Nature 425:805-811(2003).Mangel L., J. Biol. Chem. 273:30466-30471(1998).Imbert G., Nat. Genet. 14:285-291(1996).

Description

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth By similarity. Binds DNA non-specifically.Mungall A.J., Nature 425:805-811(2003).Mangel L., J. Biol. Chem. 273:30466-30471(1998).Imbert G., Nat. Genet. 14:285-291(1996).

Specification
Aliases	ARID domain-containing protein 1B; Osa homolog 2; hOsa2; p250R; BRG1-binding protein hELD/OSA1
Entrez GeneID	57492;
Swissprot	Q8NFD5
WB Predicted band size	170kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Rat
Immunogen	Synthesized peptide derived from interal of human BAF250B.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
ICC	1/100-1/500

	Western blot analysis of extracts from LOVO cells, using BAF250B antibody P42054.
	Immunofluorescence analysis of HUVEC cells, using BAF250B antibody P42054.