Rabbit Polyclonal Antibody to BCAR1

包装: 1G|100G|500G|1KG|100KG

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别名:CAS; CAS1; CASS1; CRKAS; P130Cas
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion. The molecular cloning of p130 Cas has shown it to represent a novel SH3 containing signaling molecule with a cluster of multiple putative SH2-binding motifs for v-Crk. By immunoprecipitation analysis, p130 Cas has been shown to be highly phosphorylated at tyrosine residues subsequent to either v-Src p60 or v-Crk-mediated transformation and to form stable complexes with both of these transforming proteins. p130 Cas behaves as an extremely potent substrate for protein tyrosine kinases and has been reported to relocate from the cytoplasm to cell membrane upon tyrosine phosphorylation. One proposed model is that the SH2 domain of v-Crk functions to activate c-Src kinase, which in turn phosphorylates p130 Cas.

Description

BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion. The molecular cloning of p130 Cas has shown it to represent a novel SH3 containing signaling molecule with a cluster of multiple putative SH2-binding motifs for v-Crk. By immunoprecipitation analysis, p130 Cas has been shown to be highly phosphorylated at tyrosine residues subsequent to either v-Src p60 or v-Crk-mediated transformation and to form stable complexes with both of these transforming proteins. p130 Cas behaves as an extremely potent substrate for protein tyrosine kinases and has been reported to relocate from the cytoplasm to cell membrane upon tyrosine phosphorylation. One proposed model is that the SH2 domain of v-Crk functions to activate c-Src kinase, which in turn phosphorylates p130 Cas.

Specification
Aliases	CAS; CAS1; CASS1; CRKAS; P130Cas
Swissprot	P56945
WB Predicted band size	93 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide of human BCAR1
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/200-1/1000
IHC	1/25-1/100
ELISA	1/1000-1/2000

	Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Hela cells, 231 cells, A431 cells, Primary antibody: P11236(BCAR1 Antibody) at dilution 1/100, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 4 minutes
	The image is immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using P11236(BCAR1 Antibody) at dilution 1/15. (Original magnification: ×200)
	The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P11236(BCAR1 Antibody) at dilution 1/15. (Original magnification: ×200)