Rabbit Polyclonal Antibody to Bi-Phospho-Syk(Y525/526)

包装: 1G|100G|500G|1KG|100KG

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别名:Tyrosine-protein kinase SYK, Spleen tyrosine kinase, p72-Syk, SYK
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
Syk is a positive effector of BCR-stimulated responses. This protein couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-342 and Tyr-346. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion.

Description

Syk is a positive effector of BCR-stimulated responses. This protein couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-342 and Tyr-346. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion.

Specification
Aliases	Tyrosine-protein kinase SYK, Spleen tyrosine kinase, p72-Syk, SYK
Entrez GeneID	6850
Swissprot	P43405
WB Predicted band size	72.1kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This Syk Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y525/526 of human Syk.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/250-1/1000
IHC	1/100-1/500

	Western blot analysis of lysates from Raji cell line, untreated or treated with TPA, 200nM, using (Cat. #P34398)(upper) or Tubulin(lower).
	Western blot analysis of lysates from Raji cell line, untreated or treated with TPA, 200nM, 30min, using 457167101(Cat. #P34398)(upper) or Tubulin (lower).
	Western blot analysis of anti-Phospho-Syk Pab(Cat. #P34398) in mouse liver tissue lysate (35ug/lane). Phospho-Syk(arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.