Rabbit Polyclonal Antibody to Bloom Syndrome (Phospho-Thr99)

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别名:RECQ2; RECQL3; type 2; EC 3.6.1; RecQ protein-like 3
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3′-5′ direction. Involved in 5′-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5′-ssDNA. Negatively regulates sister chromatid exchange (SCE).Ellis N.A., Cell 83:655-666(1995)Karow J.K., J. Biol. Chem. 272:30611-30614(1997)Pichierri P., EMBO J. 23:3154-3163(2004)

Description

Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3′-5′ direction. Involved in 5′-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5′-ssDNA. Negatively regulates sister chromatid exchange (SCE).Ellis N.A., Cell 83:655-666(1995)Karow J.K., J. Biol. Chem. 272:30611-30614(1997)Pichierri P., EMBO J. 23:3154-3163(2004)

Specification
Aliases	RECQ2; RECQL3; type 2; EC 3.6.1; RecQ protein-like 3
Entrez GeneID	641;
Swissprot	P54132
WB Predicted band size	159kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Peptide sequence around phosphorylation site of threonine 99 (Q-E-T(p)-Q-R) derived from Human Bloom Syndrome.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/1000
IHC	1/50-1/100

	Western blot analysis of extracts from HepG2 cells using Bloom Syndrome Protein (Phospho-Thr99) Antibody P40393.The lane on the right is treated with the antigen-specific peptide.
	Immunohistochemical analysis of paraffin-embedded human heart tissue, using Bloom Syndrome Protein (Phospho-Thr99) antibody P40393 (left)or the same antibody preincubated with blocking peptide (right).