Rabbit Polyclonal Antibody to c-Met (Ab-1003)

包装: 1G|100G|500G|1KG|100KG

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别名:EC 2.7.10.1; HGF receptor; HGF-SF receptor; Hepatocyte growth factor receptor precursor; Met proto- oncogene tyrosine kinase
应用:WB,IHC,ICC
反应种属:Human
规格：50μl/100μl

Description
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. Acts as a receptor for Listeria internalin inlB, mediating entry of the pathogen into cells.Hillier L.W., Nature 424:157-164(2003).Wang D., J. Biol. Chem. 277:36216-36222(2002).Giordano S., Nat. Cell Biol. 4:720-724(2002)

Description

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. Acts as a receptor for Listeria internalin inlB, mediating entry of the pathogen into cells.Hillier L.W., Nature 424:157-164(2003).Wang D., J. Biol. Chem. 277:36216-36222(2002).Giordano S., Nat. Cell Biol. 4:720-724(2002)

Specification
Aliases	EC 2.7.10.1; HGF receptor; HGF-SF receptor; Hepatocyte growth factor receptor precursor; Met proto- oncogene tyrosine kinase
Entrez GeneID	4233;
Swissprot	P08581
WB Predicted band size	155kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Synthesized non-phosphopeptide derived from human c-Met around the phosphorylation site of tyrosine 1003 (V-D-Y(p)-R-A).
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100
ICC	1/100-1/500

	Western blot analysis of extracts from HepG2 cells, using c-Met (Ab-1003) antibody P41660.
	Immunofluorescence analysis of A549 cells, using c-Met (Ab-1003) antibody P41660.
	Immunohistochemistry analysis of paraffin-embedded human brain tissue using c-Met (Ab-1003) antiobdy P41660.