中文 
别名:c- Myc epitope Tag应用:WB
反应种属:Fusion proteins containing the c-Myc Tag
规格：50μl/100μl
| Description |
|---|
| A c-myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A c-myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting. It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway. |
| Specification | |
|---|---|
| Aliases | c- Myc epitope Tag |
| Swissprot | P01106 |
| WB Predicted band size | 55 kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Fusion proteins containing the c-Myc Tag |
| Immunogen | Synthetic peptide: C-EQKLISEEDL conjugated with KLH. |
| Formulation | pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
| Application | |
|---|---|
| WB | 1/10000-1/50000 |
| ELISA | 1/5000-1/10000 |
 |
Gel: 8%SDS-PAGE, Lysate: 0.0016/0.008/0.032 μg, Lane 1-3: 0.0016/0.008/0.032 μg Fusion proteins containing the c-Myc Tag, Primary antibody: P00006(c-Myc Tag Antibody) at dilution 1/8000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 20 seconds |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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