Rabbit Polyclonal Antibody to CA1 (N-term)

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别名:Carbonic anhydrase 1, Carbonate dehydratase I, Carbonic anhydrase B, CAB, Carbonic anhydrase I, CA-I, CA1
应用:WB,IHC,FCM
反应种属:Human
规格：50μl/100μl

Description
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Variants of this gene have been described in some populations. Multiple alternatively spliced variants, encoding the same protein, have been identified. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature.

Description

Carbonic anhydrases (CAs) are a large family of zinc
metalloenzymes that catalyze the reversible hydration of carbon
dioxide. They participate in a variety of biological processes,
including respiration, calcification, acid-base balance, bone
resorption, and the formation of aqueous humor, cerebrospinal
fluid, saliva, and gastric acid. They show extensive diversity in
tissue distribution and in their subcellular localization. CA1 is
closely linked to CA2 and CA3 genes on chromosome 8, and it encodes
a cytosolic protein which is found at the highest level in
erythrocytes. Variants of this gene have been described in some
populations. Multiple alternatively spliced variants, encoding the
same protein, have been identified. Transcript variants of CA1
utilizing alternative polyA_sites have been described in
literature.

Specification
Aliases	Carbonic anhydrase 1, Carbonate dehydratase I, Carbonic anhydrase B, CAB, Carbonic anhydrase I, CA-I, CA1
Entrez GeneID	759
Swissprot	P00915
WB Predicted band size	28.9kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This CA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-90 amino acids from the N-terminal region of human CA1.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Application
WB	1/1000
IHC	1/100-1/500
FCM	1/10-1/50

	Western blot analysis of CA1 (arrow) using rabbit polyclonal CA1 Antibody (N-term) (Cat. #P31125). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CA1 gene.
	CA1 Antibody (N-term) (Cat. #P31125) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	CA1 Antibody (N-term) (Cat. #P31125)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CA1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.