Rabbit Polyclonal Antibody to Calmodulin

包装: 1G|100G|500G|1KG|100KG

现货支持定制

QQ咨询
| 微信咨询

400-880-2824

满200元免运费
正品保障提供发票
现货闪电当时发货

别名:Calmodulin, CaM, CALM1, CALM, CAM, CAM1
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.

Description

Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.

Specification
Aliases	Calmodulin, CaM, CALM1, CALM, CAM, CAM1
Swissprot	P62158
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This Calmodulin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-149 amino acids from the C-terminal region of human Calmodulin.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Application
WB	1/1000-1/2000
IHC	1/250

	All lanes : Anti-Calmodulin Antibody at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: Mouse brain lysate Lane 3: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	All lanes : Anti-Calmodulin Antibody at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lane 3: Mouse brain lysate Lane 4: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	All lanes : Anti-Calmodulin Antibody (C-term) at 1:1000-1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	P34460 staining hCalmodulin-D132 in human brain tissue sections by Immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/250) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
	P34460 staining hCalmodulin-D132 in human brain tissue sections by Immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/250) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.