Rabbit Polyclonal Antibody to CALR

包装: 1G|100G|500G|1KG|100KG

现货支持定制

QQ咨询
| 微信咨询

400-880-2824

满200元免运费
正品保障提供发票
现货闪电当时发货

别名:RO; CRT; SSA; cC1qR
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element.

Description

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element.

Specification
Aliases	RO; CRT; SSA; cC1qR
Swissprot	P27797
WB Predicted band size	48 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	Fusion protein of human CALR
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/1000-1/5000
IHC	1/50-1/200
ELISA	1/2000-1/10000

	Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Hela cells, 293T cells, NIH/3T3 cells, Primary antibody: P07330(CALR Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
	The image is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using P07330(CALR Antibody) at dilution 1/30. (Original magnification: ×200)
	The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using P07330(CALR Antibody) at dilution 1/30. (Original magnification: ×200)