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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to CAPZA1 (N-term)

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别名:F-actin-capping protein subunit alpha-1, CapZ alpha-1, CAPZA1
应用:WB,ICC,FCM
反应种属:Human
规格:50μl/100μl

Description
CAPZA1 is a member of the F-actin capping protein alpha subunit family. The protein regulates growth of the actin filament by capping the barbed end of growing actin filaments.
Specification
Aliases F-actin-capping protein subunit alpha-1, CapZ alpha-1, CAPZA1
Entrez GeneID 829
Swissprot P52907
WB Predicted band size 32.9kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This CAPZA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CAPZA1.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application
WB 1/1000-1/4000
ICC 1/10-1/50
FCM 1/10-1/50

All lanes : Anti-CAPZA1 Antibody (N-term) at 1:4000 dilution
Lane 1: A431 whole cell lysate
Lane 2: Jurkat whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 33 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of CAPZA1 antibody (N-term) (Cat.# P33866) in HL60 cell line lysates (35ug/lane). CAPZA1 (arrow) was detected using the purified Pab.
Immunofluorescence analysis of CAPZA1 Antibody (N-term) with paraffin-embedded human prostate carcinoma tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
CAPZA1 Antibody (N-term) (Cat. #P33866) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。